In this practical, we focused on cloning, producing, purifying, and studying the interaction between RhoX and PAK using techniques like affinity co-capture and the two-hybrid system. To simplify the process and ensure we remember the steps clearly, I created a diagram summarizing each stage, helping us grasp the workflow by week's end.
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The project explores the Sleeping Beauty (SB) transposase as an alternative to viral vectors for safer and more effective gene transfer. The improved version, hsSB, offers increased precision, particularly in CAR-T cells used to target cancers. In addition to cancer therapies, hsSB opens up possibilities for regenerative medicine. This technology combines safety and efficiency, with the potential to be combined with CRISPR/Cas for even more powerful advancements.
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Cardiac troponin detection is a game-changer in diagnosing heart attacks. Its prolonged presence in blood, detectable for up to 10 days after onset, combined with highly sensitive optical methods, offers a precise and early diagnosis. This technique holds the potential to significantly improve heart attack detection and patient care.
This presentation is based on the content: Simplifying and clearly representing a concept.